The Hepatitis B Virus Core Antibody (HBcAb) Test Kit is a rapid and convenient immunochromatographic in vitro assay for detection of HBcAb in human serum or plasma.
The test provides a visual, qualitative result, and all positive specimens are advised to be confirmed with other qualified assays.
The HBV core antigen (HBcAg) is a protein of 185 amino acids that constitutes together with the antigen e (HBeAg), the inner core of HBV. The HBcAg has a strong immunogenicity and it induces the production of antibodies (anti-HBC) that persist generally for lifetime. Anti-HBc appears shortly after the onset of infection with HBV and can usually be detected in serum soon after the appearance of circulating HBsAg and HBeAg. Testing for anti-HBc is currently being introduced at different blood banks worldwide to reduce HBV related post-transfusion hepatitis.
The principle of the HBcAb Test Kit is a competitive immunochromatographic assay. When serum is added to sample pad, it moves through the conjugate pad and dissolve the solid gold-anti-HBcAg antibody conjugate that is coated on the conjugate pad. The mixture moves along the membrane by capillary action and competes with each other to bind to HBcAg that is coated on the test region. If anti-HBcAg antibody is present, the result is no color band in T line. If there is no anti-HBcAg antibody in the sample, the T line will show a color band. The sample continues to move to the control area and forms a pink color. To serve as an internal process control, a control band should always be seen after test is completed. Absence of a colored control line in the control region is an indication of an invalid result.
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